Fingerprint analysis of composite Folii Isatidis injection by HPLC.
- Author:
Rui LIU
1
;
Zhi-gang LIU
;
Lei LI
;
Chong REN
;
Bo YUAN
;
Fa-mei LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Ginkgo biloba; chemistry; Injections; Isatis; chemistry; Plant Leaves; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Uridine; analysis
- From: China Journal of Chinese Materia Medica 2006;31(13):1059-1062
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.
METHODThe separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1). The UV detection was set at 254 nm.
RESULTA standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established.
CONCLUSIONThis method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection.
