Determination of salvianolic acid B and paeoniflorin in Shuangdan granules by HPLC.
- Author:
Zhi-wei GE
1
;
Qing HE
;
Wen-bo SHUI
;
Yi-yu CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Benzofurans; analysis; Bridged-Ring Compounds; analysis; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Glucosides; analysis; Monoterpenes; Paeonia; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Salvia miltiorrhiza; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(13):1062-1064
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for the determination of salvianolic acid B and paeoniflorin in Shuangdan granules.
METHODThe chromatographic separation was performed on a Zorbax SB C18 column with a linear gradient elution of phosphoric acid-water (0.05:100) and phosphoric acid-acetonitrile-methanol (0.05:4:96) was used. The flow rate was 1 mL x min(-1) and column temperature was set at 25 degrees C. The UV detection wavelength was set at 230 nm.
RESULTThe linear ranger was of 0.710-22.7 microg for salvianolic acid B and 0.103-3.30 microg for paeoniflorin. Three batches of Shuangdan granules were analyzed, and the content of salvianolic acid B ranged from 6.46-11.6 mg x g(-1) and paeoniflorin ranged from 1.44-1.78 mg x g(-1).
CONCLUSIONThe method was accurate, reproducible, reliable, and could be used for quantitative control of Shuangdan granules.
