Determination of scopolamine and atropine in Flos Daturae by RP-HPLC.
- Author:
Shi-guo HOU
1
;
Xue-xin GU
;
Shu-yan WANG
;
Hong-xia LI
Author Information
- Publication Type:Journal Article
- MeSH: Atropine; analysis; Chromatography, High Pressure Liquid; methods; Datura; chemistry; Flowers; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Scopolamine Hydrobromide; analysis
- From: China Journal of Chinese Materia Medica 2006;31(13):1065-1067
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC.
METHODThe two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature.
RESULTThe mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine.
CONCLUSIONThis method was simple, accurate and sensitive.
