Determination of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets by HPLC.
- Author:
Guo-bing XU
1
;
Zheng-tao WANG
;
Nie PAN
;
Zhe ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; Ginsenosides; analysis; Panax; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2006;31(13):1067-1069
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablet by reverse-phase high performance liquid chromatography.
METHODA Kromacil C18 column was used with a mixture of acetonitrile and 0.05% phosphoric acid solution (20:80) as the mobile phase at a flow rate of 1.2 mL x min(-1) with the detection wavelength at 203 nm.
RESULTThe measurement proved to be linear over the range of 0.941-9.41 g for notoginsenoside R1 and 1.04-10.4 g for ginsenoside Rg1. The average recovery of this method was 97.3% and 97.9% respectively.
CONCLUSIONThe method was simple, reliable, and accurate and can be used for the quality control of this preparation.
