Genomic characteristics of coxsackievirus B1 MSH/KM9/2009 strain isolated in Yunnan, China.
- Author:
Jian-Sheng LIU
1
;
Cong-Wen SHAO
;
Yue PAN
;
Yan-Ju ZHU
;
Xin-Qiang DENG
;
Hong-Wei LIAO
;
Ya-Ling LIU
;
Shao-Hui MA
Author Information
1. Institute of Medical Biology ,Chinese Academy of Medical Sciences and Peking Union Medical College, Kuming 650118, China. ljsh3300@163.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Child, Preschool;
China;
Coxsackievirus Infections;
virology;
Enterovirus;
classification;
genetics;
isolation & purification;
Female;
Genome, Viral;
Genotype;
Humans;
Infant;
Male;
Open Reading Frames;
Phylogeny;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2013;29(3):293-298
- CountryChina
- Language:Chinese
-
Abstract:
To characterize the complete genome sequence of coxsackievirus B1 (CVB1) MSH/KM9/2009 strain isolated from Yunnan, China,2009. Eight overlapping clones covering the whole viral genome (excluding the poly-A tail) were obtained by RT-PCR and sequenced, and their nucleotide and amino acid sequences were compared with other known CVB1 strains. The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length, and contained a 741nt non-translated region (NTR) at the 5' end and a 94nt NTR at the 3' end. The entire open reading frame contained 6 549 nt, encoding a 2 183-aa polyprotein. In the coding region, there was no nucleotide deletion or insertion, but some changes of amino acid were unique. The complete genome sequence alignments showed that the CVB1 isolate MSH/KM9/2009 strain shared the highest nucleotide (80.9%, 81.6%, 80.5% and 80%) and amino acid (95.6%, 95.8%, 96.2% and 95.6%) identities to the CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain, respectively. Phylogenetic tree analysis showed that the MSH/KM9/2009, CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain clustered into same group. The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.
