Sequence analysis of VP1 gene of the duck hepatitis A virus type 3 strains isolated from Shandong Province of China in 2012.
- Author:
Qian XU
1
;
Lin-lin CHEN
2
;
Rui-hua ZHANG
2
;
Lei YANG
2
;
Zhi-jing XIE
2
;
Yan-li ZHU
2
;
Shi-jin JIANG
2
;
Xing-kui SI
3
Author Information
1. College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China. sdauxuqian@163.com
2. College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
3. Department of Animal Medicine, Foshan Science and Technology University, Foshan 528231, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Capsid Proteins;
chemistry;
genetics;
China;
Ducks;
Hepatitis Virus, Duck;
classification;
genetics;
isolation & purification;
Hepatitis, Viral, Animal;
virology;
Molecular Sequence Data;
Phylogeny;
Picornaviridae Infections;
veterinary;
virology;
Poultry Diseases;
virology
- From:
Chinese Journal of Virology
2013;29(5):522-528
- CountryChina
- Language:Chinese
-
Abstract:
To reveal the genetic variation of the viral protein 1 (VP1) gene of the duck hepatitis A virus type 3 (DHAV-3), the VP1 gene of 13 virulent DHAV-3 strains isolated from Shandong province of China in 2012 were amplified by RT-PCR, sequenced and analyzed. The results showed that all the VP1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94. 6%-99.9% and amino acid identities of 95.0%-100%. The nucleotide and amino acid sequence homologies between the 13 DHAV-3 isolates and other 31 DHAV-3 reference strains were 92.5%-100% and 90. 8%-100%, respectively. Phylogenetic analysis showed that the VP1 gene of DHAV-3 had distinct geographical characteristics. Distribution of genotypes of the 44 DHAV-3 strains was as follows: except the vaccine strain B63, all the other Chinese isolates belonged to genotype I (GI), Vietnamese wild isolates mainly belonged to subtype 1 (S1) of genotype II (GII), and all Korean isolates belonged to subtype 2 (S2) of GII.
