Development of A synthetic positive control for the nucleic acid detection of mumps virus.
- Author:
Ai-li CUI
1
;
Li JIN
2
;
Wen-bo XU
3
Author Information
1. National Institute of Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing 102206, China. cuiaili123456@163.com
2. Virus Reference Department, Public Health England, UK, London, NW9 5EQ.
3. National Institute of Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
DNA Primers;
genetics;
standards;
Genotype;
Humans;
Mumps;
diagnosis;
virology;
Mumps virus;
genetics;
isolation & purification;
RNA, Viral;
genetics;
Reference Standards;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
standards
- From:
Chinese Journal of Virology
2013;29(5):544-547
- CountryChina
- Language:Chinese
-
Abstract:
To rapidly identify the cross-contamination problems caused by the positive control in the process of mumps virus nucleic acid detection, a new mumps virus RNA positive control was developed in this study. Using the same primers and reaction conditions, the cross-contamination problems caused by the positive control could be readily identified by comparing the fragments lengths of the PCR products between the positive control and the samples. This new RNA positive control of mumps virus can be widely used in the diagnosis and genotyping of mumps virus as a better laboratory quality control.
