Characterization of fibrillous inclusion body (FIB) of human adenovirus type 41 (Ad41) using immunoelectron microscopy.
- Author:
Jing-Dong SONG
1
;
Xiao-Hui ZOU
2
;
Min WANG
2
;
Jian-Guo QU
2
;
Zhuo-Zhuang LU
2
;
Tao HONG
2
Author Information
1. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. sjdccdc@163.com
2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
- Publication Type:Journal Article
- MeSH:
Adenovirus Infections, Human;
virology;
Adenoviruses, Human;
genetics;
metabolism;
ultrastructure;
Animals;
Female;
Humans;
Inclusion Bodies, Viral;
ultrastructure;
Mice;
Mice, Inbred BALB C;
Microscopy, Immunoelectron
- From:
Chinese Journal of Virology
2013;29(6):596-601
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the components of fibrillous inclusion body (FIB), which was formed in packaging cells during the replication of human adenovirus type 41 (Ad41), Ad41 long fiber knob (LFK) and short fiber knob (SFK) proteins were expressed in prokaryote respectively and then used to immunize BALI mice for preparation of anti-LFK serum and anti-SFK sera. The activity and specificity of anti-LFK and an ti-SFK sera were confirmed with Western blot, indirect immunofluorescence assay (IFA) and immunonegative staining, suggesting these sera could be applied in immuno-colloidal gold labelling electron microscopy (EM). 293TE cells were infected with wild-type Ad41. Ultrathin sections of infected cells were made, and labelled with immuno-colloidal gold technique using anti-Ad41 sera, anti-LFK sera, anti-SFK sera, or anti-fiber monoclonal antibody 4D2, respectively. The labelled sections were observed under EM, and the results demonstrated that both Ad41 long fiber protein and short fiber protein were included FIB.
