Isolation and characterization of rotavirus from bat.
- Author:
Le-Le XIA
1
;
Biao HE
2
;
Ting-Song HU
3
;
Wen-Dong ZHANG
4
;
Yi-Yin WANG
3
;
Lin XU
2
;
Nan LI
2
;
Wei QIU
3
;
Jing YU
3
;
Quan-Shui FAN
3
;
Fu-Qiang ZHANG
3
;
Chang-Chun TU
5
Author Information
1. College of Animal Science and Technology, Jilin Agriculturral University, Changchun 130118, China. xialele6@163.com
2. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
3. Center for Disease Control and Prevention, Chengdu Military Region of PLA, Kunming 650118, China.
4. Center for Animal Disease Control and Prevention of Yunnan Province, Kunming 650051, China
5. College of Animal Science and Technology, Jilin Agriculturral University, Changchun 130118, China.
- Publication Type:Journal Article
- MeSH:
Animals;
China;
Chiroptera;
virology;
Humans;
Molecular Sequence Data;
Phylogeny;
Rotavirus;
classification;
genetics;
isolation & purification;
ultrastructure;
Rotavirus Infections;
veterinary;
virology;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2013;29(6):632-637
- CountryChina
- Language:Chinese
-
Abstract:
Bats are considered as important animal reservoirs for many pathogenic viruses to humans. The viral metagenomic analysis was performed to study gut and lung tissues of 30 insectivorous bats collected in Yunnan Province and 26 reads were noted to group A rotavirus (RVA). Further RT-PCR screening on bat samples and in vitro viral isolation on cell cultures confirmed the presence of a novel RVA, named as RVA/Bat-tc/MYAS33/2013/G3P[10], in one of 30 Stoliczka's trident bats. The VP7 gene of this strain MYAS33 was closely related to that of an equine RVA strain from Argentina and the nucleotide sequence similarity was 93%, while its VP4 gene was a rare P[10] type and obtained the maximum sequence identity (94.8%) with that of a human strain from Thailand. The present study highlights the potential role of bats as reservoirs for RVAs.
