Research progress of real-time quantitative PCR method for group A rotavirus detection.
- Author:
Yan-Qing GUO
1
;
Dan-Di LI
2
;
Zhao-Jun DUAN
2
Author Information
1. Department of Viral Diarrheal, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. qing9041@126.com
2. Department of Viral Diarrheal, Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Humans;
Real-Time Polymerase Chain Reaction;
methods;
trends;
Rotavirus;
classification;
genetics;
isolation & purification;
Rotavirus Infections;
diagnosis;
virology;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2013;29(6):651-654
- CountryChina
- Language:Chinese
-
Abstract:
Group A rotavirus is one of the most significant etiological agents which causes acute gastroenteritis among infants and young children worldwide. So far, several method which includes electron microscopy (EM), enzyme immunoassay (EIA), reverse transcription-polymerase chain reaction (RT-PCR)and Real-time Quantitative PCR has been established for the detection of rotavirus. Compared with other methods, Real-time quantitative PCR have advantages in specificity, sensitivity, genotyping and quantitative accuracy. This article shows a overview of the application of real-time quantitative PCR technique to detecte group A rotavirus.
