IL15 DNA adjuvant enhances cellular and humoral immune responses induced by DNA and adenoviral vectors encoding HIV-1 subtype B gp160 gene.
- Author:
Ke XU
;
Shao-Hua XU
;
Xia FENG
;
Shuang-Qing YU
;
Yi ZENG
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Adjuvants, Immunologic;
Animals;
Antibodies, Viral;
immunology;
Antibody Specificity;
Female;
Genetic Vectors;
genetics;
HIV Envelope Protein gp120;
immunology;
HIV Envelope Protein gp160;
genetics;
immunology;
HIV Envelope Protein gp41;
immunology;
Humans;
Immunity, Cellular;
Immunity, Humoral;
Interleukin-15;
genetics;
Mice;
Mice, Inbred BALB C;
Vaccines, DNA;
genetics;
immunology
- From:
Chinese Journal of Virology
2014;30(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
To enhance the immunogenicity of DNA and adenoviral vector vaccines expressing HIV-1 subtype B gp160, human interleukin 15 (hIL15) DNA adjuvant (pVR-hIL15) was constructed. BALB/c mice received DNA prime/protein boost immunization with pVR-HIVgp160/Ad5-HIVgp160 alone or combined with pVR-hIL15. Cellular and humoral immune responses were evaluated by IFN-gamma enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay, respectively. Compared with those immunized with vaccines alone, the mice immunized with vaccines combined with pVR-hIL15 had significantly increased specific cellular response and antibody titer (P < 0.05). It suggests that the IL15 DNA adjuvant can enhance the immune responses induced by prime-boost regimen using DNA and adenoviral vector encoding HIV-1 subtype B gp160.
