Molecular epidemiological characteristics of HIV-1 strains isolated from newly diagnosed MSM subjects (2006-2010) in Beijing, China.
- Author:
Jing-Rong YE
;
Wan-Chun ZANG
;
Xue-Li SU
;
Hong-Yan LU
;
Ming-Qiang HAO
;
Ruo-Lei XIN
;
Guo-Min CHEN
;
Xiong HE
;
Yi ZENG
- Publication Type:Journal Article
- MeSH:
Adult;
China;
epidemiology;
Epidemics;
Genetic Variation;
HIV Infections;
diagnosis;
epidemiology;
virology;
HIV-1;
classification;
genetics;
isolation & purification;
Homosexuality, Male;
statistics & numerical data;
Humans;
Male;
Middle Aged;
Molecular Epidemiology;
Molecular Sequence Data;
Phylogeny;
Young Adult
- From:
Chinese Journal of Virology
2014;30(2):138-142
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to analyze the molecular epidemiological characteristics of HIV-1 strains prevailing among men who have sex with men (MSM) in Beijing, China. The pol gene fragments from 250 newly diagnosed HIV-1-infected MSM individuals during 2006-2010 in Beijing were amplified by RT-nested PCR, sequenced, and phylogenetically analyzed. HIV-1 pol gene from 189 individuals were amplified and analyzed; 81 (42. 9%), 3 (1. 6%), 2 (1.0%), 88 (46. 6%), and 15 (7.9%) individuals were infected with HIV-1 subtypes B, B', C, CRF01_AE, and CRF07_BC, respectively. The subtypes B and CRF01_AE could both be grouped into two clusters, and CRFO7_BC strains shared high homology and were presumed to originate from a common ancestor. The HIV-1 circulating in MSM in Beijing had a lower genetic diversity than in heterosexuals. The HIV-1 epidemic (2006-2010) in MSM in Beijing was actually a rapid spread of HIV-1 CRF01 AE and B, or rather native strains of the two viruses.
