Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein.
- Author:
Xin-Jun LV
;
Xin-Xin SHEN
;
Peng-Cheng YU
;
Hao LI
;
Li-Hua WANG
;
Qing TANG
;
Guo-Dong LIANG
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Antibodies, Viral;
immunology;
Epitope Mapping;
Epitopes, B-Lymphocyte;
chemistry;
genetics;
immunology;
Female;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Molecular Sequence Data;
Nucleoproteins;
chemistry;
genetics;
immunology;
Rabies;
immunology;
virology;
Rabies virus;
chemistry;
genetics;
immunology
- From:
Chinese Journal of Virology
2014;30(3):253-256
- CountryChina
- Language:Chinese
-
Abstract:
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
