Genome amplification and sequence analysis of human bocavirus 2.
- Author:
Huan-Di CUI
;
Yu JIN
;
Guang-Cheng XIE
;
Wei-Xia CHENG
;
Zhao-Jun DUAN
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Gene Amplification;
Genome, Viral;
Human bocavirus;
chemistry;
classification;
genetics;
Humans;
Molecular Sequence Data;
Nucleic Acid Conformation;
Parvoviridae Infections;
virology;
Phylogeny;
RNA, Viral;
chemistry;
genetics;
Terminal Repeat Sequences
- From:
Chinese Journal of Virology
2014;30(3):257-262
- CountryChina
- Language:Chinese
-
Abstract:
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
