Application of immunomagnetic screening strategy for separation of CD4+ and CD8+ T cell subpopulations of peripheral blood.
- Author:
Meng-Jie FENG
1
;
Chen QIU
;
Ying-Jun LAI
;
Cai-Xia CHEN
;
Fu-Rong LI
Author Information
1. Department of Respiratory Medicine, The Second Affiliated Hospital, Medical College of Ji'nan University, Shenzhen 518020, China.
- Publication Type:Journal Article
- MeSH:
CD4-Positive T-Lymphocytes;
cytology;
CD8-Positive T-Lymphocytes;
cytology;
Flow Cytometry;
Humans;
Immunomagnetic Separation;
methods;
Leukocytes, Mononuclear;
cytology;
immunology
- From:
Journal of Experimental Hematology
2005;13(2):205-209
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the separation of T lymphocyte subsets by immunomagnetic beads and to find optimization of strategy for specific binding of antibody-coated beads to cells, two strategies to isolate enriched T lymphocyte subpopulation CD4+ T cells and CD8+ T cells from small volumes (< 5 ml) of peripheral blood by using immunomagnetic beads or complement cytotoxicity method were compared. The purity and activity of CD4+ T cells and CD8+ T cells were measured by using flow cytometry, trypan-blue dye exclusion test, etc. The results showed that the yields of CD4+ T lymphocytes and CD8+ T lymphocytes by using immunomagnetic beads were (94.2 +/- 1.4)% and (93.8 +/- 3.0)% respectively, higher than those of control group and the group of using completement cytotoxicity method (P < 0.05). At the same time, the yields of CD4+ T lymphocytes and CD8+ T lymphocytes by using complement cytotoxicity method were (76.0 +/- 2.8)% and (77.0 +/- 3.0)% respectively, higher than those of unenriched group (P < 0.05). The trypan-blue dye exclusion test confirmed that there were no influences on activity of CD4+ T cells and CD8+ T cells when immunomagnetic beads were used for separation of these cells from peripheral blood. It is concluded that the immunomagnetic bead method has a higher efficiency for separation of CD4+ T cells and CD8+ T cells from peripheral blood than complement cytotoxic method, especially for small sample. This method has no influence on activity and proliferation of T lymphocyte subpopulations, and would be expected to establish conditions for research of biological characteristics of CD4+ T cells and CD8+ T cells in future.
