Polymorphism of HLA-B* 40 gene family in Chinese Han population.
- Author:
Zhen LI
1
;
Shi-Zheng JIN
;
Liang-Hong CHENG
;
Da-Ming WANG
;
Dan ZHOU
;
Hong-Yan ZOU
;
Guo-Guang WU
Author Information
1. Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518035, China. shallylizhen@sahu.com
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
Blood Donors;
China;
Gene Frequency;
Genotype;
HLA-B Antigens;
genetics;
HLA-B40 Antigen;
Humans;
Polymerase Chain Reaction;
methods;
Polymorphism, Genetic
- From:
Journal of Experimental Hematology
2005;13(2):215-218
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the allele distribution of HLA-B* 40 gene family in Chinese Han population and to study its influence on the selection of clinical transplantation donor, the HLA-B genetypes of 381 individuals randomly selected from Chinese National Marrow Donor Project were identified by PCR-SSO, and then all the HLA-B* 40 positive samples from the above population and the B* 40 homozygote samples received from another 1 270 registered donors were analyzed by PCR-SBT and PCR-SSP at high resolution. The results showed that the population of 381 registered donors was examined at HLA-B locus by using Hardy-Weinberg equilibrium, the gene frequency of HLA-B* 40 was 0.1692. Four different HLA-B* 40 alleles (B* 4001, B* 4002, B* 4003, B* 4006) were identified, and the serological specificity was B60 and B61 respectively. The relative frequency of each allele was 0.1192 for B* 4001, 0.0154 for B* 4002, 0.0038 for B* 4003, 0.0308 for B* 4006. The distribution of B* 40 homozygote revealed a certain regularity at high-resolution, B* 40XX (B* 4001 group), at low-resolution; B* 4001 at high resolution; B* 40XX (B* 4002 group), at low-resolution; B* 4002 or B* 4006 or heterozygote of both at high-resolution. It is concluded that in Chinese Han population, predominant allele in HLA-B* 40 gene family is B* 4001, the high-resolution typing may be recommended to use for the selection of clinical transplantation donor.
