Sequence analysis of 3'-non-coding region of RHD.
- Author:
Chao-Peng SHAO
1
;
Chun-Hui HE
Author Information
1. Sequence Shenzhen Blood Center, Shenzhen 518035, China.
- Publication Type:Journal Article
- MeSH:
3' Untranslated Regions;
genetics;
Base Sequence;
Humans;
Molecular Sequence Data;
Rh-Hr Blood-Group System;
genetics;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2005;13(2):219-221
- CountryChina
- Language:Chinese
-
Abstract:
There are 3' non-coding region, downstream Rhesus box, SMP1 gene et cetera. after RHD stop code. This study was intended to determine the sequence of 3' non-coding region. One pairs of primer was designed and then a polymerase chain reaction (PCR) was established for specific amplification of whole length of 3' non-coding region of RHD in 10 Rh-positive and 10 D(el) samples. The PCR products were purified and directly sequenced. The results showed that all Rh-positive and D(el) samples were identical, which revealed that there were 103 bp between 3'-end of RHD coding region and 5'-end of downstream Rhesus box. The D(el) samples showed the same result with the normal Rh-positive sample. It suggests that lower expression of D antigen in D(el) red cells does not associate with 3' non-coding region of D(el) gene.
