Proliferation and apoptosis in vitro of umbilical cord blood CD34+CD38- hematopoietic early progenitor cells.
- Author:
Hong TIAN
1
;
Shi-Ang HUANG
;
Fei-Li GONG
;
Jin-E ZHENG
;
Yan-Li HE
;
Jing YANG
;
Zhong CHEN
Author Information
1. Department of Medical Biology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
ADP-ribosyl Cyclase 1;
analysis;
Adult;
Antigens, CD34;
analysis;
Apoptosis;
Cell Proliferation;
Cells, Cultured;
Female;
Fetal Blood;
cytology;
immunology;
Flow Cytometry;
Hematopoietic Stem Cells;
cytology;
immunology;
Humans
- From:
Journal of Experimental Hematology
2005;13(2):229-234
- CountryChina
- Language:Chinese
-
Abstract:
To cultivate CD34(+)CD38(-) cells isolated from umbilical cord blood of healthy puerperal women over a longer-period of time for observation of cell division, proliferation, apoptosis, and effects of stem cell factor on the growth of CD34(+)CD38(-) cells, with flow cytometry, CD34(+)CD38(-) cells were isolated from umbilical cord blood of 10 healthy puerperal women and cultivated in stem cell media with supplement of IL-3, IL-6, GM-CSF, EPO, IGF-1 and SCF 6 kinds cell growth stimulating factors for six months. The cell growth curves were established. The effects of stem cell factor on the growth of CD34(+)CD38(-) cells and cell apoptosis were investigated with the single cell gel electrophoresis technique and flow cytometry method, respectively. The results showed that CD34(+)CD38(-) cells isolated from umbilical cord blood were capable of proliferating after being cultivated in vitro over a longer-period of time with no evidence of the presence of excessive apoptosis. In conclusion, under appropriate culture conditions, CD34(+)CD38(-) hematopoietic early progenitor cells from umbilical cord blood can serve as a resource providing a large amount of primitive cells for transplantation therapy after a longer period of cultivation and proliferation in vitro.
