Influence of cytochrome C on apoptosis induced by daunorubicine in acute myeloid leukemia (AML) cells.
- Author:
Ri-Ling CHEN
1
;
Ming-Zhen CHEN
;
Kang-Rong CAI
;
Xi-Min FANG
;
Zhong-Lü YE
Author Information
1. Department of Pediatrics, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China. CRL319@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Antibiotics, Antineoplastic;
pharmacology;
Apoptosis;
drug effects;
Cytochromes c;
pharmacology;
Daunorubicin;
pharmacology;
Dose-Response Relationship, Drug;
Drug Synergism;
Flow Cytometry;
Humans;
Leukemia, Myeloid, Acute;
pathology;
Microscopy, Fluorescence;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2005;13(2):282-285
- CountryChina
- Language:Chinese
-
Abstract:
The purpose was to study the responses of AML cell treated with cytochrome C and to explore the influence of cytochrome C on apoptosis of AML cell induced by daunorudicine (DNR). The differentiation of AML cell was detected by Wright-Giemsa staining and NBT test, the apoptosis of AML cell was assayed by flow cytometry and fluorescence microscopy. The results showed as follows: (1) different concentrations of cytochrome C could induce different effects on AML cells. Concentration of cytochrome C for differentiation was 10 microl/ml, for apoptosis was 20 microl/ml, and for necrosis was 40 microl/ml. (2) the apoptosis of AML cells decreased with the administration of cytochrome C in 10.0 microg/ml before treating AML cells with DNR (P < 0.01), but no change was shown with the administration of cytochrome C in 20.0 microg/ml (P > 0.05). (3) in reverse sequence, administrating of cytochrome C in 10 microl/ml and 20 microl/ml after treating AML cells with DNR, two different concentrations of cytochrome C could increase the apoptosis of AML cells (P < 0.01). It is suggested that cytochrome C may probably affect the apoptosis of AML cells induced by DNR.
