Establishment of cytokine-independent human myelodysplastic cell line and its characteristics.
- Author:
Ze-Ye SHAO
1
;
Bao-An CHEN
;
Guo-Hua XIA
;
Meng XUE
;
Chong GAO
;
Jia-Hua DING
;
Yun-Yu SUN
;
Jun WANG
;
Jian CHENG
;
Gang ZHAO
;
Xue-Zhi GAO
Author Information
1. Institute of MDS, Department of Hematology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD19;
analysis;
Antigens, CD20;
analysis;
Apoptosis;
drug effects;
Bone Marrow Cells;
metabolism;
pathology;
ultrastructure;
CD79 Antigens;
analysis;
Cell Differentiation;
drug effects;
Cell Line;
Cell Proliferation;
drug effects;
Culture Media;
pharmacology;
Cytokines;
pharmacology;
Flow Cytometry;
HLA-DR Antigens;
analysis;
Humans;
Lewis X Antigen;
analysis;
Male;
Microscopy, Electron, Scanning;
Microscopy, Electron, Transmission;
Middle Aged;
Myelodysplastic Syndromes;
metabolism;
pathology;
Sialic Acid Binding Ig-like Lectin 2;
analysis;
Time Factors
- From:
Journal of Experimental Hematology
2005;13(2):298-303
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish a cytokine-independent human myelodysplastic cells line from bone marrow of a patient with MDS-CMML. This cell line was incubated in mixed culture of RPMI 1640 and DMEM with 15% bovine serum, but without cytokines; its biological characteristics were identified by morphology, surface marker profiles, cell proliferation, differentiation and apoptosis. The results showed that the established cell line could not depend on cytokines for long-term survival and growth, and could differentiate into colony-forming unit-macrophage, colony-forming unit-megakaryocyte. In conclusion, a cytokine-independent human myelodysplastic syndrome cell line, named MDS-JSN04 (MDS Nanjing Jiansu 04), was established. Its partial biological characteristics were identified and clarified.