Preparation of transfusable human universal red blood cell with recombinant alpha-galactosidase.
- Author:
Feng GONG
1
;
Qiu-Shuang LÜ
;
Ying YOU
;
Hong-Wei GAO
;
Guo-Qiang BAO
;
Xin GAO
;
Su-Bo LI
;
Li-Li LI
;
Ying-Li WANG
;
Shu-Guang TIAN
;
Zhi-Xin ZHANG
;
Ping ZHANG
;
Yang-Pei ZHANG
Author Information
1. Department of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
classification;
immunology;
Blood Group Incompatibility;
prevention & control;
Blood Transfusion;
methods;
Coffee;
enzymology;
Electrophoresis, Polyacrylamide Gel;
Enzyme-Linked Immunosorbent Assay;
Erythrocytes;
immunology;
metabolism;
Humans;
Isoantigens;
drug effects;
metabolism;
Recombinant Proteins;
metabolism;
pharmacology;
alpha-Galactosidase;
genetics;
metabolism;
pharmacology
- From:
Journal of Experimental Hematology
2005;13(2):313-316
- CountryChina
- Language:Chinese
-
Abstract:
In order to meet the demand for safe transfusion in special conditions and to utilize the donated blood supply efficiently, technology has been developed to convert erythrocytes from type A, B, or AB to "universal donor" blood. Conversion of blood type B to O was performed by means of recombinant alpha-galactosidase digestion. The results showed that blood type B to O was converted successfully, 1 transfusion unit of red cells of group B (100 ml totally) could converted to universal blood cells in the optimal conditions including pH 5.6, 26 degrees C, 2 hours, obturation and sterilization. It is concluded that the universal red blood cells converted from group B to group O are conformed to demand of identification rules of biological products, no harmful effects of alpha-galactosidase on cell structure and function are observed. The converted red cells can stored in 4 degrees C for 21 days.
