Role of Bcl-xL in the cathepsin D-associated apoptosis of K562 cells.
- Author:
Ying PIAO
1
;
Li-Mei LIU
;
Xie-Qun CHEN
;
Rong LIANG
;
Gao-Sheng HUANG
;
Yan QIAO
;
Ai-Qing WANG
;
Zhe WANG
Author Information
1. Department of Hematology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
physiology;
BH3 Interacting Domain Death Agonist Protein;
metabolism;
Blotting, Western;
Cathepsin D;
metabolism;
Cytochromes c;
metabolism;
Fluorescent Antibody Technique;
Glucosamine;
pharmacology;
Humans;
K562 Cells;
bcl-2-Associated X Protein;
metabolism;
bcl-X Protein;
metabolism;
physiology
- From:
Journal of Experimental Hematology
2005;13(3):379-382
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of study was to explore the possible functions of Bcl-xL in the glucosamine sulfate-induced apoptosis of chronic myeloid leukemia K562 cells. Light microscopy and Wright-Giemsa staining were used to investigate the morphologic evidences for apoptosis of K562 cells induced by glucosamine sulfate (GS); immunofluorescence was used to observe the translocation of cathepsin D and cytochrome C during the apoptosis; Western blot was performed to detect the expression of Bcl-xL, Bid, Bax in K562 cells treated by GS. The results showed that many vacuoles were observed in the cytoplasma of the K562 cells treated by GS; fluorescent signals of cathepsin D and cytochrome were fransformed from granules to disperse form by using immunofluorescence; the expression of Bcl-xL was found down-regulated in K562 cells treated by GS, but not in the cells pre-treated with pepstatin A; the significant changes were not detected in expression of Bax and Bid protein before or after apoptosis. It is concluded that Bcl-xL protein may mediate relationship between cathepsin D and mitochondia pathway, Cathepsin D may play an important role in the GS inducing apoptosis of K562 cells through downregulation of Bcl-xL expression.
