Damage effect of hepatocytic growth-promoting substance from neonatal calf on DNA of HL-60 cells.
- Author:
Hong-Li ZHU
1
;
Xue-Chun LU
;
Hui FAN
;
Hong XIN
;
Xiao-Meng ZHUANG
;
Yang YANG
;
Shan-Qian YAO
Author Information
1. Department of Gerontological Hematology, General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Apoptosis;
drug effects;
genetics;
Cattle;
Cell Proliferation;
drug effects;
Comet Assay;
DNA Damage;
DNA, Neoplasm;
analysis;
genetics;
HL-60 Cells;
Humans;
Peptides;
pharmacology
- From:
Journal of Experimental Hematology
2005;13(3):383-385
- CountryChina
- Language:Chinese
-
Abstract:
The present study was aimed to investigate the damage effect of hepatocyte growrh promoting substance (HGS) on the HL-60 cell DNA in vitro and to explore the possible mechanism underlying the effect. Experiment was divided into 3 groups: one was control group, in which 0.9% NaCl solution was added, and other two were experimental group 1 and group 2, where 22.5 microg/ml and 40 microg/ml HGS were added, respectively. HL-60 cell growths were compared between groups with and without HGS. Single cell gel electrophoresis (SCGE) was used to detect DNA damage of HL-60 cell. DNA electrophoresis was used to detect the apoptosis of HL-60 cells caused by HGS. The results showed that the inhibitory effects of HGS on growth of HL-60 cells were observed in group with 22.5 microg/ml and group with 40 microg/ml after culture for 2 days, the DNA ladder and the apoptosis of HL-60 cells occurred in these 2 groups on day 2 after addition of HGS, the counts of HL-60 cells with comet tail in these experimental groups were found to be more increased in comparison with control group. In conclusion, the HGS can inhibit the growth of HL-60 cell and the apoptosis of HL-60 cells should be induced through pathway of DNA damage caused by HGS.
