Effect of realgar on expression of survivin in leukemia cell lines and its significance.
- Author:
Yan-Feng XIAO
1
;
Ya LIU
;
Shan-Xi LIU
;
Li-Fen REN
Author Information
1. Department of Pediatrics, The Second Hospital, Xi'an Jioatong University, Xi'an 710004, China. xiaoyanfeng0639@sina.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Blotting, Western;
Caspase 3;
metabolism;
Dose-Response Relationship, Drug;
Fluorescent Antibody Technique;
HL-60 Cells;
Humans;
Immunohistochemistry;
Inhibitor of Apoptosis Proteins;
Jurkat Cells;
Leukemia;
metabolism;
pathology;
Microtubule-Associated Proteins;
biosynthesis;
Neoplasm Proteins;
biosynthesis;
Sulfides;
pharmacology;
Time Factors;
fas Receptor;
metabolism
- From:
Journal of Experimental Hematology
2005;13(3):386-390
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of realgar on expression of survivin in leukemia cell lines, HL-60 and Jurket cell lines were used as in vitro models. The expression of survivin was detected by Western blot analysis and immunofluorescence, and the expressions of Fas and caspase-3 were examined by immunohistochemistry. The results showed that the expression of survivin was positive in the two cell lines. HL-60 cells did not express Fas and caspase-3, and Jurket cells were Fas-positive and caspase-3 was negative. Realgar induced a dose- and time-dependent down-regulation of survivin expression in Jurket cells, and especially in HL-60. Caspase-3 expression changed from negative to positive in HL-60 cell, but there still was no expression in Jurket cell. It is concluded that survivin expression level decreased during leukemia cell apoptosis induced by Realgar. The down-regulation of survivin expression may be an important mechanism in leukemia cell apoptosis induced by realgar through mitochondrial pathway.
