Study on bone marrow transplantation camouflaged with methoxy polyethylene glycol.
- Author:
Hui LONG
1
;
Suo-Qin TANG
;
Xiao-Fei ZHANG
Author Information
1. Department of Pediatrics, General Hospital of Chinese PLA, Beijing, 100853, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Transplantation;
adverse effects;
methods;
Female;
Graft vs Host Disease;
etiology;
mortality;
prevention & control;
Leukocyte Count;
Male;
Mice;
Mice, Inbred BALB C;
Polyethylene Glycols;
therapeutic use;
Random Allocation;
Survival Analysis;
Survival Rate;
Transplantation, Homologous;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2005;13(3):408-411
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of bone marrow camouflaged with methoxy polyethylene glycol (mPEG) on allogeneic bone marrow transplantation, 60 BALB/c(H-2d) mice were randomly divided into 3 groups after irradiation by 8.0 Gy of (60)Co gamma ray. A group was given RPMI 1640 0.5 ml in tail vein. B group was infused with the bone marrow cells (1 x 10(7)) mixed with the spleen cells (1 x 10(7)) of donor 615(H-2k) mice. C group was transplanted with same dose cells, which were camouflaged with mPEG before infusion. Severity GVHD was determined by total manifestation of mice, survival rate, survival time and histo-pathological microscopy, and engraftment of allogeneic bone marrow was evaluated by chromosome examination. The results showed that 75% mice in B group had severe adverse manifestations, such as hunched posture, diarrhea and loss of hair. Occurrence of the same adverse manifestations in C group was 35% and significantly lower than that in B group (P
