Establishment of a transplantable human myeloid BALB/c nude mouse model.
- Author:
Ya-Ming WEI
1
;
Ji-Hong LIN
;
Rong XIA
;
Jun-Cai LAN
Author Information
1. Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. weiyaming@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD;
blood;
Antigens, Differentiation, Myelomonocytic;
blood;
Antineoplastic Agents, Alkylating;
pharmacology;
CD13 Antigens;
blood;
Cyclophosphamide;
pharmacology;
Female;
Flow Cytometry;
Fusion Proteins, bcr-abl;
genetics;
Gene Expression Regulation, Leukemic;
drug effects;
Humans;
K562 Cells;
Leukemia, Experimental;
blood;
genetics;
pathology;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
blood;
genetics;
pathology;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Neoplasm Transplantation;
RNA, Messenger;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Sialic Acid Binding Ig-like Lectin 3;
Transplantation, Heterologous
- From:
Journal of Experimental Hematology
2005;13(4):596-600
- CountryChina
- Language:English
-
Abstract:
To establish a mouse model bearing transplantable human chronic myeloid leukemia for hematopoietic stem cell transplantation to treat leukemia, 4 - 5-week-old female BALB/c nude mice were given cyclophosphamide 2 mg/mouse at day -2, -1, and then the human chronic myeloid leukemia K562 cells were engrafted into the mice at day 0 by injection via tail vein or peritoneal cavity. PB and BM cells were collected, the CD45, CD13, and CD33 antigens were delected by using FCM, the bcr/abl fusion gene mRNA was examined by RT-PCR. The results showed that transplantable leukemic mice could be yielded from 4 - 5-week-old nude mice either by injection through tail vein or peritoneal cavity when the total number of inoculated tumor cells was more than 2 x 10(5) per mouse, whether being pretreated with 2 mg CTX/mouse or not. The transplanted mice could survive 30 - 60 day with leukemia. In conclusion, the mouse model bearing leukemia can be established by inoculation 2 x 10(5) K562 cells into immunodeficient BALB/c nude mice.
