Effect of IL-15 on the proliferation, differentiation and anti-apoptosis of CD34+ cells in patients with MDS.
- Author:
Ming-Zheng CHENG
1
;
Zhong-Lu YE
;
Kang-Rong CAI
;
Xiu-Lan HUANG
;
Ri-Ling CHENG
;
Han-Rong CHEN
Author Information
1. Department of Pediatrics, Affiliated Hospital of Guangdong Medical College, Zhangjiang 524001, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
immunology;
Antigens, CD19;
immunology;
Antigens, CD34;
immunology;
Antigens, Differentiation, Myelomonocytic;
immunology;
Apoptosis;
drug effects;
Bone Marrow Cells;
drug effects;
immunology;
pathology;
Cell Cycle;
drug effects;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Flow Cytometry;
Humans;
Interleukin-15;
pharmacology;
Microscopy, Fluorescence;
Myelodysplastic Syndromes;
blood;
immunology;
pathology;
Receptors, Transferrin;
immunology;
Sialic Acid Binding Ig-like Lectin 3
- From:
Journal of Experimental Hematology
2005;13(4):620-623
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of interleukin-15 (IL-15) on the proliferation, differentiation and apoptosis of MDS CD34(+) cells, CD34(+) cells of high enrichment were separated by MACS system, and cultured in liquid media with different concentration of IL-15 in treated group and without IL-15 in the control group. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and cell by FCM, and the other MDS CD34(+) cells were stained by cytochemical staining after culture. The results showed that after culture with IL-15 the proliferation and differentiation of MDS CD34(+) cells were obviously promoted. It was found the every lineage of mature cells developed, the expressions of cell surface antigens CD71, CD33 and CD19 all increased in the MDS CD34(+) cell treated with IL-15. It is suggested that IL-15 stimulates the proliferation and differentiation of MDS CD34(+) cells, and partly shows anti-apoptosis effects which may be applicable to the therapy MDS.
