Selective depletion of donor alloreactive T cells by using immuno-magnetic cell sorting.
- Author:
Hu LÜ
1
;
Yi-Huan CHAI
;
Jie XU
Author Information
1. Department of Hematology, Children Hospital Affiliated to Suzhou University, Suzhou 215003, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
immunology;
Antigens, Differentiation, T-Lymphocyte;
immunology;
Bone Marrow Transplantation;
adverse effects;
methods;
Flow Cytometry;
Graft vs Host Disease;
immunology;
prevention & control;
Humans;
Immunomagnetic Separation;
methods;
Interleukin-2 Receptor alpha Subunit;
immunology;
Lectins, C-Type;
T-Lymphocytes;
cytology;
immunology
- From:
Journal of Experimental Hematology
2005;13(4):637-640
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore a new method of alleviating graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation through selective elimination of human alloreactive T cells expressing either CD25(+) or CD69(+) by immuno-magnetic cell sorting (MACS). Healthy donor peripheral blood mononuclear cells were cocultivated with bone marrow mononuclear cells from HLA-nonidentical leukemia recipient with remission in one-way mixed lymphocyte culture (OWMLC). After 3 days, both CD25(+) and CD69(+) lymphocytes were removed by MACS. The depleted donor fraction and untreated donor cells were then rechallenged in a secondary mixed lymphocyte culture (MLC) with the original stimulator cells or a third party to assess relative alloreactivity. The results showed that 50% inhibition of the secondary MLC was observed in the depleted donor fraction. Alloreactivity against unrelated third-party cells was largely preserved. It is concluded that this method reduces alloreactivity while retaining reactivity against a third party target in vitro.
