Voltage-dependent potassium channel and calcium-activated potassium channel current changes of peripheral blood T-lymphocytes from hypertensive patients in Xinjiang Kazakh
10.3760/cma.j.issn.0253-3758.2013.12.007
- VernacularTitle:新疆哈萨克族高血压病患者外周血T淋巴细胞电压依赖性钾通道及钙激活钾通道的电流变化
- Author:
Qiu-Bing ZHANG
1
;
Yuan-Ming ZHANG
;
Lu-Feng CHENG
;
Qing-Yan YUAN
;
Gui-Ming ZHANG
;
Ping LIANG
;
Fang GOU
Author Information
1. 新疆医科大学第一附属医院心脏中心
- Keywords:
Hypertension;
T-lymphocytes;
Kv1.3 potassium channel;
Potassium channels,calcium-activated
- From:
Chinese Journal of Cardiology
2013;41(12):1020-1024
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the current changes of voltage-dependent potassium channel (Kv1.3 potassium channel) and calcium-activated potassium channel (IKCa1 potassium channel) in peripheral blood T-lymphocyte derived from hypertensive patients of Xinjiang Kazakh.Methods Twenty randomly selected untreated Kazakh hypertensive patients and 20 Kazakh healthy subjects from Xinjiang were included in this study.T-lymphocytes were isolated from peripheral blood with magnetic cell sorting,the whole-cell currents of Kv1.3 and IKCa1 potassium channels were recorded with patch-clamp technique.Results (1) The current density of Kv1.3 potassium channel was significantly higher in the hypertensive group [(280 ±74) pA/pF (n =39)] than that in the control group [(179 ±51) pA/pF (n =38),P ＜ 0.01],while the membrane capacitance was similar between the two groups.(2) The current density of IKCa1 potassium channel was also significantly higher in the hypertensive group [(198 ± 44) pA/pF (n =28)] than that in the control group [(124 ± 43) pA/pF (n =26),P ＜ 0.01],while the membrane capacitance was also similar between the two groups.Conclusions The T-lymphocytes Kv1.3 potassium channel and IKCa1 potassium channel current densities are higher in hypertensive patients in Xinjiang Kazakh suggesting a potential role of Kv1.3 and IKCa1 potassium channels activation in the pathophysiology of hypertension.
