The stability of the atherosclerotic plaque depends on the extent of injured endothelium: results from a novel model of ischemia/reperfusion induced atherosclerosis in carotid artery of rats
10.3760/cma.j.issn.0253-3758.2013.12.012
- VernacularTitle:血管内皮损伤程度决定动脉硬化斑块的稳定性缺血再灌注动脉硬化模型的建立
- Author:
Xue-Qing JIN
1
;
Xu-Wei WU
;
Zhuo-Qiang LU
;
Jing-Jing GONG
;
Hua-Jun WANG
;
Chang-Sheng XU
Author Information
1. 福建医科大学附属第一医院心内科 福建省高血压研究所
- Keywords:
Arteriosclerosis;
Endothelium,vascular;
Reperfusion injury
- From:
Chinese Journal of Cardiology
2013;41(12):1040-1044
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the atherogenic lesion progress in a novel ischemia/reperfusion induced atherosclerosis model in the carotid artery of rats.Methods Rats were divided into normal control,sham-operated control and ischemia-reperfusion injury(IRI) groups (n =10 each).IRI was induced by 30 min carotid artery occlusion with a 2 cm long artery clips in anesthetized rats.Four weeks later,hematoxylin and eosin (HE) and immunohistochemical stain were performed on carotid arteries of various groups.The ratio of neointima area/media area (I/M) and expression of platelet endothelial cell adhesion molecule (PECAM-1/CD31) were compared among groups.Results (1) Neointimal hyperplasia was detected in carotid artery of IRI group and the I/M ratio was significantly higher than in normal control and shamoperated groups (1.328 ±0.301 vs.0.011 ±0.004 and 0.017 ±0.008,all P ＜0.01).(2) Small to largesized neointima were found in the IRI group and the small sized intima was stable while large sized intima which covered the whole cavity was instable and underwent spontaneous rupture and thrombosis formation.(3) CD31 expression was significantly upregulated in carotid artery of IRI group corresponding to the instability of neointima in this group.Conclusion Ischemia-reperfusion injury of carotid artery could result in atheroma in rats,this model could be used for future research on the pathogenesis of atherosclerosis.Our results show that endothelium injury of the arteries is the key factor to trigger atheroma and responsible for the disruption of the plaque.
