Protective effects of soybean isoflavone on human umbilical vein endothelial cell injury induced by H2O2 and lipopolysaccharide
10.3760/cma.j.issn.0253-3758.2014.02.011
- VernacularTitle:大豆异黄酮对过氧化氢及脂多糖诱导的血管内皮细胞损伤保护机制的实验研究
- Author:
Yan WANG
1
;
Weiping BU
;
Hong XIE
;
Aihua QU
;
Jun LIU
Author Information
1. 第四军医大学第二附属医院疼痛生物研究所
- Keywords:
Endothelial cells;
Isoflavones;
Hydrogen peroxide;
Lipopolysaccharides
- From:
Chinese Journal of Cardiology
2014;42(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects and related mechanisms of soybean isoflavone (SI) on human umbilical vein endothelial cells (HUVECs) injury induced by H2O2 and lipopolysaccharide (LPS).Methods H2O2 and LPS were used to induce HUVECs injury in vitro.Nine experimental groups were examined:control group,H2O2 (2 mmol/L for 4 h),LPS (2 mmol/L for 4 h),H2O2 + low dose SI (1 mg/ml),H2O2 + moderate dose SI (2.5 mg/ml),H2O2 + high dose SI (5 mg/ml),LPS + low dose SI (1 mg/ml),LPS + moderate dose SI (2.5 mg/ml),LPS + high dose SI (5 mg/ml).The survival ratio of HUVECs was detected with MTT assay.The cultured cells were loaded by Fura-2/AM and the change of [Ca2+] in HUVECs was measured by fluorospectrophotometry.The contents of malondialdehyde (MDA),superoxide dismutase (SOD),reduced glutathione (GSH-Px) were measured by the commercial kits.The levels of tissue plasminogen activator IL-6 in the supematant were measured by enzyme linked immunosorbent assay (ELISA) kits.Apoptosis rate of the HUVECs was analyzed by flow cytometry.Results H2O2 and LPS significantly decreased HUVECs viability,increased the contents of MDA,IL-6 and decreased the contents of SOD and GSH-Px,and increased the apoptosis rate [(37.8 ± 1.8) ％ and (38.9 ± 1.1) ％].Co-treatment with SI could reduce MDA and IL-6 while increase SOD and GSH-Px and reduce apoptosis in a dose-dependent manner.Conclusion The findings demonstrate that soybean isoflavone could attenuate H2O2 and LPS induced injury in human umbilical vein endothelial cells through protecting mitochondrial function,improving antioxygenic activity,and suppressing the mobilization of cvtosolic calcium.
