Platelet derived growth factor receptor β over-expression in endothelial progenitor cells promote reendothelialization after vascular injury
10.3760/cma.j.issn.0253-3758.2014.03.008
- VernacularTitle:过表达血小板衍生生长因子受体β的小鼠内皮祖细胞有助于损伤后血管的再内皮化
- Author:
Hang WANG
1
;
Hao HUANG
;
Yangguang YIN
;
Mengyang DENG
;
Huali KANG
;
Lan HUANG
Author Information
1. 430070,广州军区武汉总医院干部病房二科
- Keywords:
Endothelium,vascular;
Stem cells;
Receptor,platelet-derived growth factor beta
- From:
Chinese Journal of Cardiology
2014;42(3):214-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of platelet derived growth factor receptor β (PDGFR-β) transfected endothelial progenitor cells (EPCs) on vascular regeneration.Methods Spleen-derived mononuclear cells (MNCs) were isolated using density gradient centrifugation and induced with special culture medium.EPCs transfection was performed with LipofectamineTM 2000 reagent according to the instruction manual.Carotid artery injury was induced in splenectomized mice.EPCs were injected by tail vein immediately and at 24 h after endothelial injury of the carotid artery.Evans blue staining was performed to evaluate reendothelialization at 7 days after endothelial injury of the carotid artery.Results Most adherent cells were LDL and UEA-I double positive.Laser scanning confocal microscopy showed that transfection efficiency was about 50％-60％.The reendothelialized area in the PDGFR-β-EPCs group was significantly larger than that in EGFP-EPCs group.Conclusion Transplantation of PDGFR-β over-expressed EPCs can promote reendothelialization in the early phase after carotid artery injury.
