The effect of formalin-induced pain on CCK in rat spinal cord neurons.
- Author:
Li LI
1
;
Hong-an LI
;
Jin-fang JIANG
;
Wei-hua LIANG
;
Jun-qiang SI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cholecystokinin; metabolism; physiology; Female; Formaldehyde; Male; Neurons; metabolism; Pain; chemically induced; physiopathology; Random Allocation; Rats; Rats, Wistar; Receptors, Cholecystokinin; metabolism; physiology; Spinal Cord; metabolism; pathology
- From: Chinese Journal of Applied Physiology 2009;25(2):217-220
- CountryChina
- Language:Chinese
-
Abstract:
AIMCCK is one of the strongest endogenous anti-opioid substances and suppresses morphine tolerance which results from long term use of morphine. This study explores the modulatory effect of CCK on pain formalin-induced.
METHODSThe effect of formalin-induced pain on CCK immunoreactivity in rat sensory neurons was observed through immunohistochemistry technique.
RESULTSAfter 1 h of subcutaneous injection of formalin in one paw of rats, the number of positive neurons of CCK immunoreactivity in spinal cord neurons was obviously increased and greater than that of non-injection side (P <0.01). The semi-quantitative optical density average values of CCK immunoreactivity neurons were 0.397 +/- 0.014 and 0.295 +/- 0.007 in injection side and non-injection side respectively, the difference was obvious (P < 0.01). After 3 h of subcutaneous injection of formalin in one paw of rats, the semi-quantitative optical density average values of CCK immunoreactivity neurons were 0.366 +/- 0.009 and 0.303 +/- 0.005 in injection side and noninjection side respectively, the difference was significant (P < 0.01).
CONCLUSIONFormalin-induced pain can significantly change semi-quantitative optical density average value of CCK immunoractivity in spinal cord neurons, this indicates CCK participates in modulation of pain.
