The effects of urea on ECG and the sodium currents of ventricular myocyte in mice.
- Author:
Xue-xin ZHANG
1
;
Shu-gang XI
;
Chun-yan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arrhythmias, Cardiac; physiopathology; Electrocardiography; Female; Heart Ventricles; cytology; Male; Membrane Transport Proteins; genetics; Mice; Mice, Inbred C57BL; Mice, Knockout; Myocytes, Cardiac; metabolism; physiology; Patch-Clamp Techniques; Sodium Channels; metabolism; physiology; Urea; metabolism
- From: Chinese Journal of Applied Physiology 2009;25(2):224-227
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the effects of urea on ECG and sodium currents of ventricular myocyte in mice.
METHODSECG and patch clamp techniques were used in the experiments, to record ECG of mice and sodium currents of ventricular myocyte in mice.
RESULTSUrea could lead mice heart rate evidently slow down (P < 0.01) with concentration dependent. The heart rate were (556 +/- 29, 469 +/- 37, 378 +/- 48) b minT in low, middle, high groups respectively before using urea and (612 +/- 27, 615 +/- 23, 619 +/- 26) x min(-1) after. The conduction block arrhythmia was happened in middle and high groups. The sodium currents of ventricular myocyte in mice was inhibited by urea(P < 0.05). The sodium currents amplitude value were reduced to (7.32 +/- 0.68, 5.69 +/- 0.64, 4.58 +/- 0.57) nA after using urea in each group and were (8.76 +/- 0.91, 8.87 +/- 1.01, 8.77 +/- 0.96) nA before, submit concentration dependent.
CONCLUSIONUrea can inhibit the sodium currents of ventricular myocyte in mice to make it happen conduction block arrhythmia.
