Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats.
- Author:
Yuan-yuan LV
1
;
Biao SUN
;
Ji-zheng MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cardiomegaly; etiology; metabolism; Constriction; Male; Proteome; analysis; metabolism; Proteomics; methods; Random Allocation; Rats; Rats, Sprague-Dawley; Renal Artery; physiopathology
- From: Chinese Journal of Applied Physiology 2009;25(2):237-242
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation.
METHODS16 male SD rats were separated into 2 groups (n=8): 2 kidney 1 Clip group (2K1C) and sham operation group (SO). The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
RESULTSOverall, 21 protein spots showed significant difference, and 14 out of which were identified.
CONCLUSIONKidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.
