Involvement of leukotrine B4 receptors in the inflammatory responses and immunological regulation in vitro.
- Author:
Chun-guang HAN
1
;
Huo-gao HUANG
;
Ming HU
;
Wen-yan LUO
;
Yue GAO
;
Qiong WANG
;
Yong-xue LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cell Proliferation; drug effects; Fatty Alcohols; pharmacology; Glycols; pharmacology; Inflammation; immunology; Male; Rats; Rats, Wistar; Receptors, Leukotriene B4; antagonists & inhibitors; physiology; Synovial Membrane; cytology; immunology; Tetrazoles; pharmacology; Th1-Th2 Balance
- From: Chinese Journal of Applied Physiology 2009;25(2):273-276
- CountryChina
- Language:Chinese
-
Abstract:
AIMBLT1 and BLT2 were both recently cloned and identified as two subtypes of leukotrine B4 (LTB4) receptors. With the usage of U-75302 and LY255283, the specific antagonists of BLT1 and BLT2 respectively, the involvement of BLT1 and BLT2 in the inflammatory and immunological responses was in vitro explored.
METHODS(1) To investigate inhibition of U-75302 and LY255283 on the proliferation of rat synovial cells, 3H-TdR incorporation into the cells was quantified. (2) Flow cytometric assay for interferon-gamma (IFN-gamma) and interleukine 4 (IL-4) profiles in CD4+ T lymphocytes from rat spleen was carried out to determine the ratio of Th1/Th2.
RESULTS(1) For inhibition on rat synovial cells proliferation, U-75302 exerted its effect only at a high concentration of 10 micromol/L and LY255283 at the concentrations of 10 micromol/L-10 micromol/L. (2) Both U-75302 and LY255283 could elevate the percentage of Th2, but could not influence that of Th1.
CONCLUSIONBLT1 and BLT2 were involved in the synovial cells proliferation change the ratio of Th1/Th2. Their meaning served as targets for prevention and treatment of infectious diseases should be emphasized.
