Dynamic changes of Smac/DIABLO protein expression in rat donor myocardium suffered from hypothermic preservation.
- Author:
Ming-zhi ZHENG
1
;
Jian-ping JIANG
;
Lin FU
;
Li ZHU
;
Ying-ying CHEN
;
Yue-liang SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carrier Proteins; metabolism; Cold Temperature; Heart; Heart Transplantation; Male; Mitochondrial Proteins; metabolism; Myocardium; metabolism; Organ Preservation; methods; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2009;25(3):296-300
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of different duration of hypothermic preservation on the expression of Smac/DIABLO protein in rat hearts.
METHODSThe Langendorff model of isolated rat heart was used. After stored in 4 degrees C Celsior solution for different time (0, 3, 6, 9 or 12 h), the activity of SOD and the content of MDA in heart mitochondria were measured. Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein was also analyzed by Western blotting.
RESULTS(1) After hypothermic preservation, the activity of SOD was decreased and the content of MDA was increased in rat hearts in a time-dependent manner. (2) Prolonged the hypothermic preservation, the percentage of apoptotic cell also enhanced. (3) After long-term of cold preservation, the expression of Smac/DIABLO protein increased at 3-6 h of preservation but decreased after 9 h.
CONCLUSIONProlonged the hypothermic preservation might lead to the expression of Smac/DIABLO protein and induce cardiomyocytes apoptosis, which may in turn result in malfunction of cardiomyocytes.
