Cardiovascular effects of intermedin1-53 and its mechanism.
- Author:
Jing-hui YANG
1
;
Yong-fen QI
;
Cun-gen MA
;
Chao-shu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenomedullin; metabolism; pharmacology; Animals; Blood Pressure; drug effects; Cardiovascular Physiological Phenomena; drug effects; Cyclic AMP; metabolism; Heart; drug effects; In Vitro Techniques; Male; Neuropeptides; metabolism; pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley; Ventricular Function; drug effects
- From: Chinese Journal of Applied Physiology 2009;25(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
AIMThe present study was designed to determined the cardiovascular effects of IMD1-53 in rats and its possible mechanism.
METHODSIsolated rat hearts were perfused by Iangendorff mode, and ventricular function was measured after IMD1-53 perfusion. Meanwhere, we investigated the effects of IMDI) on arterial pressure after intravenous administration of IMD. And cAMP content was detected in rat ventricular and aortic tissues.
RESULTSThe results showed that perfusion with IMD significantly enhanced cardiac function and resulted in higher LVSP, +dp/dt(max) and -dp/dt(max) by 45%, 51% and 37%, respectively, compared with control and increased coronary infusion flow. The effects of IMD1-53 on cardiac function were antagonized by H-89, an inhibitor of PKA. The content of cAMP in the ventricular tissues after IMD perfusion was 131% higher than control. In addition, intravenous administration of IMD induced a potent decrease in arterial pressureand heart rate, and in aortic tissues, IMD incubation resulted in a 236% increase in cAMP content compared with control group.
CONCLUSIONThe study reveals that IMD can increase cardiac function and decrease arterial pressure in rat and the effects may be related to cAMP pathway.
