Heart-type fatty acid-binding protein alteration in rat's cardiocyte after acute myocardial infarction.
- Author:
Hong-yan WANG
1
;
Xiao-yun ZHAO
;
Fa-zhong YANG
;
Han-ying XING
;
Xue GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Down-Regulation; Fatty Acid-Binding Proteins; genetics; metabolism; Male; Myocardial Infarction; metabolism; Myocytes, Cardiac; metabolism; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2009;25(3):312-315
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the changes of mRNA and protein expressions of heart-type fatty acid binding protein (H-FABP) in rat ischemic myocardium at different intervals ischemia.
METHODS60 SD male rats weighing 250-350 g, were randomly divided into one sham-operated group and five study groups (group A1, A2, A3, A4, A5, the left coronary artery of rats has been ligated for 1 h, 2 h, 4 h, 6 h, 12 h respectively). Myocardil samples from infarct zone, ischemic and non-ischemic zone, were obtained for histology examination, and the mRNA for H-FABP in ischemic myocardial tissue were determined by RT-PCR. Serum free fatty acid(FFA) was determined by colorimetric method.
RESULTSCompared to sham hearts, H-FABP mRNA expression were significantly decreased in ischemia zone of AMI rat hearts (P < 0.05), especially in rats underwent 4 h ischemia and 6 h ischemia (P < 0.01). Serum FFA were significantly increased in AMI rats relative to sham rats (P < 0.05).
CONCLUSIONSignificant down-regulated heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder.
