The effect of danshen on the angiogenesis of the frozen-thawed human fetal ovarian tissue after transplantation.
- Author:
Dan-dan WU
1
;
Ying ZHOU
;
Bing-sen XU
;
Yue-hong TONG
;
Lin-ju
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-2; genetics; metabolism; Animals; Cryopreservation; Drugs, Chinese Herbal; pharmacology; Female; Fetal Tissue Transplantation; methods; Fetus; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neovascularization, Physiologic; drug effects; Ovarian Follicle; cytology; growth & development; transplantation; RNA, Messenger; genetics; metabolism; Salvia miltiorrhiza; chemistry; Transplantation, Heterologous; methods; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2009;25(3):330-333
- CountryChina
- Language:Chinese
-
Abstract:
AIM(1) To investigate the mRNA expression of the key angiogenic growth factors in the grafts after transplantation. (2) To investigate the potential impact of danshen (Chinese traditional medicine) administration on grafts angiogenesis.
METHODSThe frozen-thawed ovarian tissue from aborted fetus were xenografted into the renal capsule of the nude mice, recovered 48 h, 7 d and 28 d after respectively. Either danshen or saline (as the control) was administered after transplantation.
RESULTSThe mRNA levels of VEGF showed a temporary raise in 48 h after transplantation, then decreased in one week, and no significant difference was fund between the control group and danshen group. Ang-2 was increased in 48 h after transplantation, when Danshen group was significantly higher than the control group (P < 0.05). The microvessel density significantly increased in all the tissues after transplantation. The control group peaked on day 7 after transplantation, while danshen group peaked in 48 h and kept correspondingly steady after that.
CONCLUSIONEarly angiogenesis began within 48 h after transplantation of the thawed human fetal ovarian tissue, and its microvessel density peaked within the first week after transplantation. Our results also suggested that the use of danshen injection in conjunction with transplantation could facilitate revascularization of the grafts.
