Effects of human HSF1 on transcriptional regulation of prohibitin promoter.
- Author:
Xiao-hua LIU
1
;
Dong-sheng GUO
;
Xin-xing WANG
;
Zhi-qing ZHANG
;
Rui ZHAN
;
Xiao-ming WANG
;
Ling-jia QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA-Binding Proteins; physiology; Gene Expression Regulation; HEK293 Cells; Heat Shock Transcription Factors; Humans; Molecular Sequence Data; Promoter Regions, Genetic; Repressor Proteins; genetics; Transcription Factors; physiology; Transcription, Genetic; Transfection
- From: Chinese Journal of Applied Physiology 2009;25(3):349-352
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
METHODSThe total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
RESULTSThe pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.
CONCLUSIONhHSF1 can transcriptionally regulate prohibitin expression.
