Effect of liver X receptors agonists T0901317 on expression of FAT/CD36 gene mRNA in adult human skeletal muscle cell.
- Author:
Rong ZENG
1
;
Ya-lian SA
;
Xin-min YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; CD36 Antigens; genetics; metabolism; Cells, Cultured; Female; Humans; Hydrocarbons, Fluorinated; pharmacology; Liver X Receptors; Male; Muscle, Skeletal; cytology; metabolism; Orphan Nuclear Receptors; agonists; RNA, Messenger; genetics; metabolism; Sulfonamides; pharmacology
- From: Chinese Journal of Applied Physiology 2009;25(3):428-432
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the liver X receptors agonists T0901317's effect on expression of FAT/CD36 gene mRNA in adult human skeletal muscle cell.
METHODSMyotubes from humans were exposed to different T0901317 concentrations (0, 0.5, and 1.0 micromol/L) for 24 hours before experiments were performed. Then the expression of FAT/CD36 mRNA in skeletal muscle cell of each experimental group was detected by SYBR Green I real-time quantitative polymerase chain reaction. The relative data were compared among groups by 2-delta delta Ct method.
RESULTS(1) The Ct mean of control group, T0901317 (0.5 micromol/L) group, T0901317 (1 micromol/L) group were analyzed and there was significant difference (P < 0.01). (2) The expression of FAT/CD36 mRNA with liver X receptors agonists T0901317 in human skeletal muscle cell in the T0901317 (0.5 micromol/L) group and T0901317 (1 micromol/L) group were 2.91 times and 3.03 times than the control group.
CONCLUSIONThe expression of FAT/CD36 mRNA in human skeletal muscle cell afer the treatment of liver X receptors agonists T0901317 is increased, so we may propose that T0901317 may increase the risk of resistance in adult human skeletal muscle.
