Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia.
- Author:
Jian BAI
1
;
Ge CHI
;
Jun ZHANG
;
Li-na LIU
;
Miao FAN
;
Hai-dong LIU
;
Wei-wei LI
;
Jing YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carnosine; pharmacology; Cell Hypoxia; Cells, Cultured; Endothelial Cells; pathology; Female; Free Radical Scavengers; pharmacology; Male; Protective Agents; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2010;26(1):30-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of camosine on the injury of rat vascular endothelial cells(VECs) induced by hypoxia.
METHODSThe model of the injury of rat VECs induced by hypoxia was established. The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay. The levels of lactate dehydrogenase (LDH) activity in cell medium were measured with corresponding kit. The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.
RESULTSAfter culturing VECs with camosine (10 to 20 mmol/L) for 6 hours, the decrease in VECs activity induced by 12 and 24 hour hypoxia was inhibited. The release of LDH was also inhibited, and the integrity of cell structure remained.
CONCLUSIONCamosine has the protective effect on hypoxia injured VECs.
