Carbon monoxide inhalation protects against limb ischemia/reperfusion injury.
- Author:
Jing LI
1
;
Zhen-long ZHU
;
Wan-zheng QI
;
Zhong-li SHI
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Inhalation; Animals; Carbon Monoxide; pharmacology; Creatine Kinase; metabolism; Extremities; blood supply; Femoral Artery; Ischemia; physiopathology; L-Lactate Dehydrogenase; metabolism; Male; Proto-Oncogene Proteins c-bcl-2; metabolism; Rats; Rats, Sprague-Dawley; Reperfusion Injury; prevention & control; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Applied Physiology 2010;26(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of carbon monoxide(CO) inhalation in the limb ischemia/reperfusion (I/R) injury of rats.
METHODSForty-four Sprague-Dawley rats were randomly divided into three groups: S, I/R and RC groups. I/R injury model was made by the occlusion of the femoral artery for four hours and the reperfusion for forty-eight hours. RC group was exposed to medical air mixed CO (the volume fraction of CO: 0.05%) during limb reperfusion in rats. The other two groups were exposed to the routine air. Gross morphology of the ischemic limb, wet-to-dry weight ratio (W/D), and skeletal muscle histopathology were observed. The apoptosis index and expression levels of Bax and Bcl-2 in the muscle were assessed with Flow Cytometry. The activities of serum lactate dehydrogenase (LDH) and creatine kinase (CK) were tested by Automatic Biochemical Analyzer.
RESULTSCompared to I/R group, W/D, serum LDH and CK activities, the apoptosis index and Bax expression level in the muscle were all significantly decreased, the Bcl-2 expression level was significantly increased, gross morphology of the ischemic limb and muscle histopathology were obviously improved in RC group.
CONCLUSIONInhaling exogenous CO can attenuate limb I/R injury.
