Determination of twelve ginsenosides in Panax ginseng by HPLC.
- Author:
Xiuying XU
1
;
Yimin ZHENG
;
Shanquan FU
;
Ying ZHAO
;
Jie LI
;
Linlin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Ginsenosides; analysis; Panax; chemistry; Plant Extracts; analysis; Plant Roots; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2011;36(11):1463-1465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC.
METHODThe analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm.
RESULTTwelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%).
CONCLUSIONThe method was simple,fast and could control the quality of P. ginseng effectively.
