Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC.
- Author:
Shan QIN
1
;
Xuesen WEN
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; analysis; Chlorogenic Acid; analogs & derivatives; analysis; Chromatography, High Pressure Liquid; methods; Chrysanthemum; chemistry; Cinnamates; analysis; Flowers; chemistry; Glucosides; analysis; Luteolin; analysis; Plant Extracts; analysis; Quality Control; Reproducibility of Results; Sensitivity and Specificity
- From: China Journal of Chinese Materia Medica 2011;36(11):1474-1477
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method quantitative method for simultaneous determination of chlorogenic acid, 1, 5-dicaffeoylquinic acid, isochlorogenic acid A, isochlorogenic acid C, luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Chrysanthemum morifolium Ramat.
METHODA Phenomenex Gemini-NX C18 column (4.6 mm x 250 mm, 5 microm) was used with CH3 OH and 0.4% H3PO4 as mobile phases. The flow rate was 1 mL x min(-1), the column temperature was 25 degrees C, and the detection wavelength was set at 350 nm.
RESULTThe 6 active components were in baseline separation. The linearity of this method was good (r > or = 0.999 7), and the average recoveries were 100.6% - 102.4%, RSD < 3%. Except isochlorogenic acid A, the contents of the determined components in the steam-blanched flower heads were significantly higher than those non blanched. The contents of chlorogenic acid and isochlorogenic acid A in the steam-blanched semiopened flower heads were higher than fully opened ones by 53% and 41%, respectively.
CONCLUSIONThe method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Chrysanthemum.
