Simultaneous determination of 7 alkaloid in herba Sophorae Alopecuroidis by HPLC.
- Author:
Ling MA
1
;
Junqing WANG
;
Jie TIAN
;
Kun WANG
;
Wei LI
;
Yinghua WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Piperidines; analysis; Quality Control; Quinolizidines; analysis; Quinolizines; analysis; Sophora; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(11):1483-1486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determination of 7 alkaloid in Herba Sophorae Alopecuroidis by HPLC.
METHODX-Brige C18 (4.6 mm x 200 mm, 5 microm) column was used with acetonitriles-0.05 mol x L(-1) KH2PO4 solution (2.0 mL x L(-1) triethylamine) with gradient elution as the mobile phase and 1.0 mL x min(-1) as the flow rate. The detection wavelength was 205 nm.
RESULTAloperin curve was linear in the range from 20.66 to 103.32 microg (r = 0.998 8) and the average recovery was 97.12% (RSD 7. 3%); sophoridine curve was linear in the range from 22.82 to 114.12 microg (r = 0.999 7) and the average recovery was 97.47% (RSD 3.0%); oxymatrine curve was linear in the range from 25.10 to 125.52 microg (r = 0.999 1) and the average recovery was 96.21% (RSD 4.5%); oxysophocarpine curve was linear in the range from 23.88 to 119.40 microg (r = 0.997 5) and the average recovery was 94. 64% (RSD 5.2%); matrine curve was linear in the range from 5.00 to 24.99 microg (r = 0.998 6) and the average recovery was 98.04% (RSD 5.4%); sophocarping curve was linear in the range from 4.69 to 23.46 microg (r = 0.999 6) and the average recovery was 96.24 (RSD 5.8%); lehmannine curve was linear in the range from 4.60 to 23.01 microg (r = 0.997 8) and the average recovery was 101.31% (RSD 4.3%).
CONCLUSIONThe method is accurate, simple and feasible. It can be used as a quality evaluation in Herba S. Alopecuroidis.
