Modulatory effect of triptolide on differentiation of human Th17 cells.
- Author:
Pengyuan CHEN
1
;
Rui HAN
;
Qiang ZHOU
;
Hao CHENG
;
Kejian ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Inflammatory Agents, Non-Steroidal; CD4-Positive T-Lymphocytes; drug effects; metabolism; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Diterpenes; pharmacology; Dose-Response Relationship, Drug; Epoxy Compounds; pharmacology; Humans; Interferon-gamma; drug effects; metabolism; Interleukin-17; metabolism; secretion; Phenanthrenes; pharmacology; Th17 Cells; cytology; drug effects; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(11):1499-1502
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of triptolide on the differentiation of human Th17 cells.
METHODHuman peripheral blood mononuclear cells, purified CD4+ T cells and CD4+CD45RA- memory T cells were treated with various concentrations of triptolide in vitro. Cell proliferation was determined by MTT assay. Flow cytometry was used to analyze the intracellular expression of IL-17 and IFN-gamma. Cytokine production of IL-17 and IFN-gamma was measured by ELISA.
RESULTCell proliferation, intracellular expression of IL-17 and IL-17 secretion were inhibited by triptolide in a dose-dependent manner. IFN-gamma expression and production were also inhibited by triptolide.
CONCLUSIONTriptolide inhibits the differentiation of human Th17 cell. The observation may indicate at least one of the mechanisms of the immunosuppressive and anti-inflammatory effects of triptolide.
