Identification of Junci Medulla using DNA barcoding technique.
- Author:
Xiaohui PANG
1
;
Jingyuan SONG
;
Shilin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: DNA Barcoding, Taxonomic; methods; DNA, Plant; DNA, Ribosomal Spacer; Plants, Medicinal; classification; genetics
- From: China Journal of Chinese Materia Medica 2012;37(8):1097-1099
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify Junci Medulla using the ITS2 barcode.
METHODThe ITS2 regions of Juncus effuses and its closely related species were PCR amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. The Kimura 2-Parameter (K2P) distances were calculated using software MEGA 5.0. Identification analyses were performed using BLAST1, Nearest Distance, PWG Distance and neighbor-joining (NJ) methods.
RESULTThe intra-specific genetic distances of J. effuses were ranged from 0 to 0.005, which were far lower than inter-specific genetic distances between J. effuses and its closely related species (0.215-0.614). All the four methods showed that ITS2 could discriminate J. effuses from its closely related species correctly.
CONCLUSIONThe ITS2 region is an efficient barcode for authentication of Junci Medulla, and our study further confirmed the ability of ITS2 to identify traditional Chinese medicinal materials.
