Locality identification of Chinese medicinal plant Scutellaria baicalensis (Lamiaceae) population-level DNA barcoding.
- Author:
Bin ZHANG
1
;
Qingjun YUAN
;
Luqi HUANG
;
Xiaoguang LIU
;
Xiaoming LI
;
Shufang LIN
;
Meilan CHEN
;
Xiaoguang GE
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
DNA Barcoding, Taxonomic;
methods;
Haplotypes;
Medicine, Chinese Traditional;
methods;
Phylogeny;
Scutellaria baicalensis;
classification;
genetics;
Sequence Analysis, DNA
- From:
China Journal of Chinese Materia Medica
2012;37(8):1100-1106
- CountryChina
- Language:Chinese
-
Abstract:
Scutellaria baicalensis is an important traditional Chinese medicine and Scutellaria flavonoids have received worldwide attention in recent years. It is the basis of controlling quality of S. baicalensis to develop a reliable genetic marker system used to identify locality of origin. Because of the characteristics of maternal inherited and high-rate of evolution, the cpDNA intergenic spacer can effectively elucidate the degree of genetic variation in different areas of the same species (populations), which can be used as the population-level DNA barcoding to locality identify. In this study, we have used the molecular phylogeography analysis for the three cpDNA intergenic spacers atpB-rbcL, trnL-trnF and psbA-trnH of 17 wild populations from different localities, which reveals the 20 haplotypes, including 13 polymorphic sites and constitutes a shallow gene tree. The authers have divided the haplotypes of S. baicalensis into three grades of population-level DNA barcoding according to the frequence and geographic distribution: 3 highest-frequency haplotypes as area-population-level DNA barcoding, 3 haplotypes were mainly shared by 2-3 adjacent populations as region-population-level DNA barcoding, and there are also 8 unique-population haplotypes as unique-population-level DNA barcoding. The result of this study reveals that population-level DNA barcoding is a reliable genetic marker used to locality identify of S. baicalensis.
